edward.evans@ndm.ox.ac.ukwww.t-cellbiology.org/teaching Measuring thermodynamic parameters 1.S can't be measured directly 2.G and H are measured and G = H -TS 3.H can be measured in 2 ways a)calorimetry (see later) or b)van't Hoff analysis Van't Hoff analysis 1.G is measured over a range of temperature and plotted 2.The non-linear van't Hoff equation* is fitted to the data to determine H, S and C 3.The slope represents H 4.This plot is curved for macromolecular interactions as H varies with temperature 5.The curvature represents C
{}