GPC Separation Mechanism  Polymer is prepared as a dilute solution in the eluent and injected into the system  The GPC column is packed with porous beads of controlled porosity and particle size  Large molecules are not able to permeate all of the pores and have a shorter residence time in the column  Small molecules permeate deep into the porous matrix and have a long residence time in the column  Polymer molecules are separated according to molecular size, eluting largest first, smallest last
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