LOXL2 expression induces ErbB2 activation through production of 2 O 2 . (A)Production of H 2 O 2 on Matrigel was measured as an activity read-out in the 10A cont and 10A L2 cells, by using a commercially available kit and following the manufacturer's instructions (Abcam). 10A L2 cells produced higher levels of H 2 O 2 , as expected, because of LOXL2 overexpression. (B) The 200 U/ml catalase (+cat) was added to remove H 2 O 2 produced by LOXL2. Western blotting revealed that pErbB2 was decreased in 10A L2 cells treated with catalase (L2+cat) in comparison with untreated cells (L2). (C) Addition of H 2 O 2 to 10A cont cells strongly induced ErbB2 activation. Taken together, these results indicate that, by removing H 2 O 2 , we can significantly abrogate ErbB2 activation in 10A L2 cells, and addition of H 2 O 2 activated ErbB2.
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