ChIP analysis of HSF1 binding sites in murine Ttr ( A ) and Hsp70 ( B ) promoters in hippocampus and liver after in vivo celastrol treatment. ChIP-qPCR results from treatment of murine hippocampal or hepatic cells with vehicle control (white) ( n = 4) or celastrol (gray bar) ( n = 4) are shown as percentage of input. ChIP assays were performed at least in triplicate. Multivariate analysis reveals a significant effect of celastrol on HSF1-binding activity to the Ttr promoter in the hippocampus relative to that produced by DMSO treatment. There was also an increase in binding to the Hsp70 promoter in both hippocampus and liver (Student's t test): ** p
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