Stable expression of human <t>Bcl-2</t> significantly reduces NS1-induced cell death. (A) Representative Bcl-2-stable transfectants of both KLNS and ULNS cells were detected with a Bcl-2 MAb, Bcl-2 100, by immunoblotting with 5 × 10 6 cells in the presence or absence of IPTG. The position of the Bcl-2 protein corresponding to approximately 26 kDa is indicated. (B and C) The kinetics of Bcl-2 inhibition of NS1-induced cell death was measured by trypan blue exclusion for both KLNS (B) and ULNS (C) cells with 5 × 10 5 cells for each cell line. The experiments were performed twice, with no significant difference between results.
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