Corneal allogenic intrastromal ring segments (CAIRS) preparation. (A) The donor epithelium is completely removed from limbus to limbus using dry sponges. (B) The corneal center is marked using the reticule of the VisuMax 500-kHz femtosecond laser platform (Carl Zeiss Meditec, Jena, Germany). (C) Specially designed double-bladed circular trephine (Jacob CAIRS trephine; Madhu Instruments, New Delhi, India). (D) After removing endothelium, the trephine is centered on the inked mark using the reticule. (E) Two concentric cuts are made on the donor corneal rim. (F) A circular corneal allogenic intrastromal ring segment is seen, which is then bisected into two.
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