The delayed accumulation of c- jun mRNA induced by prolonged hypoxia (Hx) is dependent on the presence of HIF-1α. Autoradiograph of a Northern blot of total RNA from wild-type and HIF-1α null mEFs incubated under aerobic conditions (air [5% CO 2 ]) or exposed to hypoxia (pO 2 ≤ 0.01%) for the indicated times. The blot was probed sequentially for the mRNAs for c- jun and 28S rRNA. This finding is representative of three independent experiments.
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