Diabetic primary bone cells. ( A ) LOX is the highest expressed paralog in nondiabetic primary osteoblasts. Absolute quantification of LOX isoforms in nondiabetic primary osteoblasts. Data were calculated based on a standard curve of known transcript copy numbers and normalized to the amount of cDNA added ( n = 5). ( B ) LOX is the most highly inhibited paralog in primary diabetic osteoblasts. Data represent percent of nondiabetic controls calculated using the ΔΔCT method using GAPDH internal control. Data are pooled from three independent experiments (data are means ± SE, * p
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