The PCR sequence starts as before, with the primer annealing to the DNA fragment, allowing the DNA polymerase to attach.
{"Image DocumentName": "The PCR sequence starts as before, with the primer annealing to the DNA fragment, allowing the DNA polymerase to attach.", "Image ImageDescription": "The DNA polymerase starts to add complementary nucleotides. Eventually, a modified nucleotide will be added, which prevents addition of any further nucleotides to the DNA strand. This generates many fragments of DNA that all end in a modified nucleotide, located in different positions on the unknown strand. These fragments are read by the automated sequencer, and the unknown sequence is revealed.", "Image XResolution": "37", "Image YResolution": "37", "Image ResolutionUnit": "Pixels/Centimeter", "Image Software": "SlidePlayer", "Image YCbCrPositioning": "Centered"}