MS induces a muscle type switch to slow-twitch fibers After crush injury and MS treatment, the quadriceps was retrieved and analyzed by western blot or histology. We found that the expression of muscle proteins was increased after MS treatment. (A) Desmin nearly doubled its expression levels, and (B) a specific increase in MyH type 1 could be detected. (C) No significant difference in MyH type 2 was found by WB. (D, E, F) Staining was performed with <t>anti-myosin-heavy-chain-slowtwitch/FITC-anti-mouse-IgM</t> (green), anti-myosin-heavy-chain-fast-twitch/Cy3-antimouse-IgG (red), and DAPI (blue). When compared with intact control quadriceps, no fiber type change was found in injured muscle without MS stimulation. (E) On the other hand, a shift to type 1 fibers was verified in MS-treated samples. (F) MyH type 1 expression was up to 3-fold higher in MS-treated samples than in native muscle. (G) As expected, a relative decrease in MyH type 2 was detected. +MS indicates magnetic stimulation treatment, whereas -MS represents the unstimulated controls (* P
{"Image Orientation": "Horizontal (normal)", "Image XResolution": "96", "Image YResolution": "96", "Image ResolutionUnit": "Pixels/Inch", "Image Software": "Adobe Photoshop CS4 Windows", "Image DateTime": "2016:11:26 12:24:22", "Image Artist": "handschc", "Image ExifOffset": "184", "Thumbnail Compression": "JPEG (old-style)", "Thumbnail XResolution": "72", "Thumbnail YResolution": "72", "Thumbnail ResolutionUnit": "Pixels/Inch", "Thumbnail JPEGInterchangeFormat": "322", "Thumbnail JPEGInterchangeFormatLength": "7491", "EXIF ColorSpace": "sRGB", "EXIF ExifImageWidth": "800", "EXIF ExifImageLength": "1196"}