The two-rounds search Mascot, Phenyx and X!Tandem
{"Image DocumentName": "The two-rounds search Mascot, Phenyx and X!Tandem", "Image ImageDescription": "The identification process may be launched in 2-rounds. Each round is defined with a set of search criteria. First round searches the selected database(s) with stringent parameters, Second round searches the proteins that have passed the first round (relaxed parameters): The first round is typically run with stringent parameters on an extended database to achieve a rough isolation of proteins of the sample while keeping combinatorial explosion under control. The second round is performed with looser parameters on a limited protein database built from the potentially identified proteins. Unspecific cleavages, mutations or a wider range of posttranslational modifications can be explored during the second round. Accelerate the job when looking for many variable modifications, or unspecific cleavages. Appropriate when the first round defines stringent criteria to capture a protein ID, and the second round looks for looser peptide identifications.", "Image XResolution": "37", "Image YResolution": "37", "Image ResolutionUnit": "Pixels/Centimeter", "Image Software": "SlidePlayer", "Image YCbCrPositioning": "Centered"}