Targeted gene modification
{"Image DocumentName": "Targeted gene modification", "Image ImageDescription": "Potential applications of TILLING, RNAi, ZFN and Meganucleases. Reverse genetics and targeted gene inactivation. (Ex creation of recessive resistances to viruses with TILLING) Inactivation by KO of genes with undesirable effects (ex: synthesis of alkalo\u00efds ) Induction of allelic diversity to optimize an agronomic trait. RNAi. The technique relies on the production of transgenics. No expected toxicity. The production of a Si RNA by the host can lead to the inactivation of. an essential gene in a pest that feeds on this host, resulting in protection. of the plant against the pest. Potential applications of ZFN and Meganucleases. More performant than TILLING and RNAi as regards the diversity. of target modifications. New therapies against DNA virus infection. Claimed targeted introduction of genes (Ex: to cure a disease) Limitations. ZNF and Meganuclease technologies are work intensive and expensive. The ZFN technology does not work well in different labs. Consulted experts: A. Choulika, Cellectis; B. Dujon, Pasteur.", "Image XResolution": "37", "Image YResolution": "37", "Image ResolutionUnit": "Pixels/Centimeter", "Image Software": "SlidePlayer", "Image YCbCrPositioning": "Centered"}