Time, temperature, and serum-dependent binding of PGG β-glucan to human neutrophils and monocytes . PGG β-glucan binding to human neutrophils and monocytes was determined (A) in the presence of 2, 5, 10, 20, and 50% of serum, (B) at 10, 30, 60, and 120 min, and (C) at RT, 4 and 37°C. The MFI and percentage of BfD IV positive cells were indicated in the zebra plots, and histogram shows PGG β-glucan binding (solid) in comparison to the vehicle-control (gray filled). Data shown in each of the conditions are representative of three independent experiments performed with cells obtained from three different donors.
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