Protocol for Quantiation of Proteins using NanoOrange™ Invitrogen.  To perform a protein assay, the protein sample is simply added to the NanoOrange™
{"Image DocumentName": "Protocol for Quantiation of Proteins using NanoOrange\u2122 Invitrogen.", "Image ImageDescription": "To perform a protein assay, the protein sample is simply added to the NanoOrange\u2122 reagent in a specialized diluent and this mixture is heated at 95\u00b0 C for ten minutes. Fluorescence can be measured as soon as the mixture has cooled to room temperature. Alternatively, samples can be read up to six hours after preparation with no loss in sensitivity, as long as samples are protected from light. The NanoOrange\u2122 reagent is virtually nonfluorescent in aqueous solution, becoming strongly fluorescent at about 570\u2014590 nm upon interaction with proteins, when excited at about 470\u2014490 nm. Detection of the fluorescence using the TD-700 fluorometer equipped with a fluorescein filter kit allows protein concentrations from 10 ng/mL to 10 \u00b5g/mL to be accurately measured relative to a standard curve.", "Image XResolution": "37", "Image YResolution": "37", "Image ResolutionUnit": "Pixels/Centimeter", "Image Software": "SlidePlayer", "Image YCbCrPositioning": "Centered"}