Residual Cellular DNA Below Limit of Detection
{"Image DocumentName": "Residual Cellular DNA Below Limit of Detection", "Image ImageDescription": "Several process steps remove or degrade DNA. No residual cellular DNA detected in 5 small-scale Ad4 wild type BDS lots made using the PaxVax process. High sensitivity qPCR (non-validated) All results below the level of detection (LOD <0.13 ng/mL) Equivalent to <0.013 ng/dose (1010 vp vaccine dose) Absence of residual DNA makes DNA fragment size determination impractical. Any DNA fragments are likely to be <200 bp due to Benzonase treatment in the PaxVax process. Benzonase able to reduce DNA to 2-5 nucleotides in length. PaxVax process uses 25,000 U/L for 3 hours (exhaustive digestion) Proprietary. VRBPAC, 19th Sept 2012.", "Image XResolution": "37", "Image YResolution": "37", "Image ResolutionUnit": "Pixels/Centimeter", "Image Software": "SlidePlayer", "Image YCbCrPositioning": "Centered"}