Purification of MITF-associated complexes. ( A ) Western blot of 501Mel cell lines stably expressing Flag-HA-tagged-MITF (F-H-MITF). ( B ) The immunoprecipitated material from the soluble nuclear extract (SNE) was separated by SDS PAGE and stained with silver nitrate. F-H-MITF is indicated along with * that designates a contaminating protein seen in the control immunoprecipitations. Lane M corresponds to a molecular mass marker indicated in kDa. ( C ) Immunoblot detection of HERC2, BRG1, USP7, USP11, XRCC5, and XRCC6 in the MITF-associated complexes. ( D ) Summary of proteins and complexes interacting with MITF. Shown are the proteins found specifically in the immunopurifications of F-H-MITF classified according to their function and organisation into known complexes. DOI: http://dx.doi.org/10.7554/eLife.06857.003
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