"Figure 5: A, Representative example of the increase of resonance units following injection on biotinylated CF1-CF2 PCR products from an homozygous W1282X sample. Three injections were consecutively performed (I–III). A total amount of 60 l of 2.5 m PCR products in HBS-EP was injected. After each injection (segments ""a"" of the panel), injections of HBS-EP (segments ""b"" of the panel) and 50 mm NaOH (segments ""c"" of the panel) were performed. No blank subtraction was performed. B, Comparison of the increase of resonance units following injection on biotinylated PCR products from normal subjects (open circles), homozygous W1282X samples (open squares), or heterozygous subjects (filled circles). Insert: Characterization by agarose gel electrophoresis of biotinylated PCR products from normal subjects (a), homozygous W1282X samples (b), or heterozygous subjects (c)."
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