Results of the nested polymerase chain reaction (PCR) assay performed on the serum specimens from both patients and their dogs. Lane 1: standard DNA size marker V (Boehringer, Mannheim, Germany); lanes 2 and 3: serum #1 from man; lanes 4 and 5: serum #1 from woman; lanes 6 and 7: serum from dog #1; lanes 8 and 9: serum from dog #2; lane #10: negative control; lanes 2, 4, 6, and 8: pure DNA; and lanes 3, 5, 7, and 9: DNA diluted 1:10 in deionized water.
{}