Immunochemistry of muscles treated with rHyal- sk in the presence and absence of GET. Skeletal muscle FFPE sections were stained at 3h (d0), d7 and d14 after treatment with monoclonal antibodies specific for macrophages ( A , F4/80) and dendritic cells ( B , MHC-II; C , CD11c). Positive control samples were muscles treated with LPS. Negative control samples included skeletal muscle harvested from untreated mice. All sections were counterstained with hematoxylin and analysed by optical microscopy. 20× magnification. Scale bars, 50 μm. rHyal- sk , recombinant Hyal from Fidia Farmaceutici; bHyal, bovine Hyal from Sigma-Aldrich; Hyal + GET, electrotransfer after Hyal injection; LPS, lipopolysaccharide injection; NEG CTRL, untreated muscle.
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