Flow cytometric analysis of MSH2 was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (R1510-32, 1/50) (blue). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; red).
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