Alternate Options for PNH QC in RBCs
{"Image DocumentName": "Alternate Options for PNH QC in RBCs", "Image ImageDescription": "Step 1: Run normal RBCs with GPA and with CD59 to determine the position of normal RBCs (Type I cells) Step 2: Run normal RBCs with GPA and without CD59 to determine the position of RBCs with complete CD59 Deficiency (Type III cells) Ideally, a PNH patient with a CD59-negative PNH clone and some remaining normal CD59+ cells should be used to: optimize the voltage setting & compensation setting (a poorly compensated set up may show the type III population in the type II region) verify adequate CD59 staining on normal cells. check for the location of the CD59-negative population. The option above may be helpful in the initial validation process for the PNH assay as it is hard to get a PNH+ patient. Step 3: Run suspected PNH patient with GPA / CD59. Presence of 6.2% PNH Type III RBCs. Type III. Type II. Type I.", "Image XResolution": "37", "Image YResolution": "37", "Image ResolutionUnit": "Pixels/Centimeter", "Image Software": "SlidePlayer", "Image YCbCrPositioning": "Centered"}