Figure 1. Comparative analysis of stem cell viability in the presence of early or late developmental stage zebrafish extracts. Cell viability was evaluated by MTT proliferation assay in control and hASCs exposed to ZF1, ZF2, ZF3, ZF4, and ZF5. Each stage related extract was given at the final concentrations of 0.01, 0.1, 1, and 10 μg/ml, for 24 hours (Panel A) or 72 hours (Panel B). Data are expressed as mean ± standard deviation of three independent experiments performed in duplicate on hASCs derived from three independent subjects. Cell viability is significantly reduced in ZF4 (10 μg/ml), and ZF5 (1 and 10 μg/ml) treated cells compared with control cells after 72 hours. Statistical analysis was determined by one-way ANOVA followed by Dunnett's post-hoc test (*p < 0.05, **p < 0.01).
{}