Loading of Analytical Scale-up - Theoretical vs. Practical
{"Image DocumentName": "Loading of Analytical Scale-up - Theoretical vs. Practical", "Image ImageDescription": "Optimized Analytical.   Product (BCL2) Xbridge C8 (4.6 \u00d7 50mm) 20-50% CH3CN/NH4Ac (pH 8) 5min, 1 ml/min, 254 nm, ~1 mg/ml. 5 \u03bcl injection. Rs   Scale Up (BCL2) Xbridge C8, 4.6 \u00d7 100mm % CH3CN/NH4Ac (pH 8) 10 min, 1 ml/min, 254 nm, ~70 mg/ml. Inj Vol: 20 \u03bcl (Vth = 90 \u03bcl) For example, here optimized method gave the Rs of 3.5 for this critical band pair. Theoretical prediction by Eq.3 said we could inject 90 ul and still see separations of this critical band-pair, or touching-band separation. But in our experiment, 20 ul injection already resulted the disappearance of this critical band pair. So, what is the maximum loading of touching-band separation for this sample? This question can only be answered by analytical scale-up experiments. What is the maximum loading of touching-band separation?", "Image XResolution": "37", "Image YResolution": "37", "Image ResolutionUnit": "Pixels/Centimeter", "Image Software": "SlidePlayer", "Image YCbCrPositioning": "Centered"}