Pulsed-field gel electrophoresis demonstrating genetic relatedness of study isolates Eco2, Eco1, and Kpn1, and a representative Klebsiella pneumoniae isolate of the epidemic clone, Kpn ST258, Israel, 2008. Bacterial DNA was prepared and cleaved with 20U SpeI endonuclease (New England Biolabs, Beverly, MA, USA), followed by electrophoresis in a CHEF-DR III apparatus (Bio-Rad Laboratories, Inc., Hercules, CA, USA), as described (4). The macrorestriction patterns of the isolates were compared accor
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