Increased susceptibility of GAD65 AA 114–122 peptide-pulsed APCs to T1D NK cell-mediated recognition associates with NK cell-ILT2 expression. Degranulation of CD3 - CD56 + ILT2 + NK cells of PBMC from T1D patients, expanded with GAD65 AA 114–122 or FLU peptides, measured as CD107a cell-surface expression following stimulation with APCs, either left unpulsed or GAD65 AA 114–122 peptide-pulsed. (A) A representative experiment out of two performed is shown. K562 cells were used as positive control. The percentage of CD3 - CD56 + CD107a + NK cells (upper panel) and CD3 - CD56 + CD107a + ILT2 + (lower panel) is indicated for each condition. (B) Summary of CD3 - CD56 + CD107a + and (C) CD3 - CD56 + ILT2 + CD107a + NK cell percentage of four T1D PBMC, expanded with GAD65 AA 114–122 or FLU peptides, following stimulation with APCs, either left unpulsed (circle dots) or GAD65 AA 114–122 (GAD65)-pulsed (square dots); horizontal bars, average values are reported.
{}