(A) Coimmunoprecipitation assays were performed by immunoprecipitation (IP) with anti-c-Jun and blotting with anti-PU.1 and anti-c-Jun. To control for nuclear protein extract concentrations, levels of the nuclear protein TATA binding protein (TBP) were examined. (B) For ChIP assays, isolated lysates were immunoprecipitated with either anti-PU.1 or anti-c-Jun, followed by amplification of purified DNA fragments using primers specific for the c- Jun promoter or Mcsfr promoter or by antiacetylated H4, followed by amplification of purified DNA fragments using primers specific for the HPRT promoter.
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