HMGB1 promotes the assembly of chromatin in vitro. (A) Chromatin was assembled in vitro on linear DNA using purified histones, hNAP1, ACF, and increasing amounts of HMGB1 protein; then it was digested with MNase. The residual DNA after digestion was electrophoresed on a 1.5% agarose gel (upper panel) and quantified with PicoGreen (normalized to the reaction containing BSA, lower panel). Error bars, SD of three replicates. (B) Chromatin was assembled in the presence of a fixed amount of HMGB1 (1 µg/ml), or BSA as control, for the indicated time points. Electrophoresis (upper panel) and quantification by PicoGreen (lower panel) of the residual DNA after digestion with MNase are shown. Error bars, SD of three replicates. MW: 100 bp ladder.
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