ANRIL/β-catenin is essential for SOX2-mediated nasopharyngeal carcinoma progression. ( A ) Levels of SOX2 and β-catenin protein expression in HNE-1 and CNE2 after stably sh-SOX2 and shControl. ( B ) β-catenin protein expression analysis in HNE-1 and CNE2 with SOX2 shRNAs alone or in combination with ANRIL vector. ( C ) Schematic diagram indicated the β-catenin binding to ANRIL region by RNA IP assays. ( D ) TOP-FOP flash assay was performed to evaluate the effect of shANRIL on WNT/β-catenin pathway. ( E ) Correlation between ANRIL and β-catenin transcript determined by quantitative real-time RT-PCR in 20 human nasopharyngeal carcinoma specimens.
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