Experimental Model – dsRNA Construct The target of these construct derived siRNA are mRNA of a subunit of an enzyme involved in chlorophyll production The result is chlorotic staining of affected areas Above Left: Wildtype (Col-0) Arabidopsis Above Right: SUL under a phloem specific promoter The chlorotic staining provides for visual determination of RNA silencing functionality and successful RNA silencing signal transport (Bleached Cell = RNA silencing successful) (Green Cell = no RNA silencing)
{}