Scanning 6-frames translated genomes with a protein sequence
{"Image DocumentName": "Scanning 6-frames translated genomes with a protein sequence", "Image ImageDescription": "The mouse urate oxidase enzyme (P25688) contains an uricase domain (EC ), catalyzing the urate degradation: Urate + O(2) + H(2)O <=> 5-hydroxyisourate + H(2)O(2). Top: blastp result (protein against protein): Homo sapiens reference proteins (Refseq) scanned with urate oxidase peptidic sequence. The scan only returns weakly scoring matches (lowest E-value = 0.004). Bottom: tblastn (search translated nucleotide database using protein query) The scan returns 4 very high-scoring genome locations. Question: why did we identify very good matches with tblastn, and not with blastp ? Protein against protein (blastp) Protein against translated DNA (tblastn)", "Image XResolution": "37", "Image YResolution": "37", "Image ResolutionUnit": "Pixels/Centimeter", "Image Software": "SlidePlayer", "Image YCbCrPositioning": "Centered"}