Importance of DNA Quantitation (prior to multiplex PCR)
{"Image DocumentName": "Importance of DNA Quantitation (prior to multiplex PCR)", "Image ImageDescription": "DNA amount. (log scale) High levels of DNA create interpretation challenges (more artifacts to review) 100 ng. -A. Too much DNA. Off-scale peaks. Split peaks (+/-A) Locus-to-locus imbalance. +A. 10 ng. 2.0 ng. Well-balanced STR multiplex. 1 ng. STR Kits Work Best in This Range. 0.5 ng. 0.1 ng. Too little DNA. Heterozygote peak imbalance. Allele drop-out. Locus-to-locus imbalance ng. Stochastic effect when amplifying low levels of DNA produces allele dropout.", "Image XResolution": "37", "Image YResolution": "37", "Image ResolutionUnit": "Pixels/Centimeter", "Image Software": "SlidePlayer", "Image YCbCrPositioning": "Centered"}