Summary of in vitro primer extension and DNase I and KMnO 4 footprinting. ( A ) Sequence of P vpsL from −60 to +30. Bold and underlined A with black arrow at +1 and bold G (+3) represent the TSS determined by primer extensions; the −10 element and the −35 region are labeled and boxed in green; sequences in bold and red denote the VpsR binding site. Protection sites from DNase I footprinting and hypersensitive sites are depicted as rectangular boxes and triangles, respectively, either above (non-template) or below (template) the sequences: gray, RNAP with or without c-di-GMP or VpsR; black, RNAP with VpsR and c-di-GMP; red, VpsR with or without c-di-GMP. The open transcription bubble detected using KMnO 4 footprinting is shown as separated ssDNA from position −11 to +2 with sites of KMnO 4 cleavage indicated as purple asterisks. ( B ) Summary of positions of protection and hypersensitive sites on non-template and template strand DNA.
{"Image Orientation": "Horizontal (normal)", "Image XResolution": "400", "Image YResolution": "400", "Image ResolutionUnit": "Pixels/Inch", "Image Software": "Adobe Photoshop CC (Macintosh)", "Image DateTime": "2018:06:27 15:04:15", "Image ExifOffset": "168", "Thumbnail Compression": "JPEG (old-style)", "Thumbnail XResolution": "400", "Thumbnail YResolution": "400", "Thumbnail ResolutionUnit": "Pixels/Inch", "Thumbnail JPEGInterchangeFormat": "306", "Thumbnail JPEGInterchangeFormatLength": "4034", "EXIF ColorSpace": "sRGB", "EXIF ExifImageWidth": "2789", "EXIF ExifImageLength": "972"}