VPA increased the acetylation pattern of histone H4. Representative de-convoluted LC-MS from Group 1 (VPA/Placebo) and Group 2 (Placebo/VPA). Histone H4 was isolated from the chromatin of patient PBMCs. There were two major peaks present, 11,307.2 Da and 11,349.0 Da. These two peaks make up the majority of histone H4. Peak 11,307.2 represents the dimethylated and 11,349.0 thedimethylated/acetylated portion of histone H4. B) Quantification of the change in the histone H4 acetylation was achieved by calculating the ratio of the dimethylated/acetylated over dimethylated histone H4; this data was then averaged by group and visit. Group 1 (VPA/Placebo) had 13 patients analyzed and had group means from 0.695 (±0.080) to 0.616 (±0.121). There were 18 patients analyzed in Group 2 (Placebo/VPA)with a group mean from 0.599 (±0.096) to 0.683 (±0.090).
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