Time lapse visualisation of replication fork dynamics and terminus segregation in *E. coli*. The terminus region of the chromosome (cyan; visualised by lac repressor-fluorescent fusion proteins bound to *lac* operators) co-localises with replication forks (red focus) as it is replicated (i). All forks are disassembled at the termination area (ii – the red focus disappears) prior to chromosome segregation (iii – two cyan labelled termini appear). New replication forks are subsequently assembled (iv – a red focus in each of the daughter cells).
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