Alkylating agents Most potent mutagen for practical application, except UV.
{"Image DocumentName": "Alkylating agentsMost potent mutagen for practical application, except UV.", "Image ImageDescription": "Compounds frequently used: ethyl methanesulfonate (EMS), methyl methanesulfonate (MMS), diethylsulfate (DES), diepoxybutane (DEB), N-methyl-N -nitro-N-nitrosoguanidine (NTG, the most effective chemical mutagens, but its use in a mutation program is difficult because of its carcinogenic effects), N-methyl-N-nitroso-urea and mustard gas. Formation of alkylated bases in DNA, along with phosphotriester, purine-free sites and single-strand breaks / 7-alkylguanine is the most common product but it does not result in mutations / O6-alkyl-guanine and O4-alkylthymine are the most important premutational lesions, and as a result of pairing errors, mainly AT\u2192GC transitions are elicited (direct mutagenesis). Transitions, transversions, deletions and frameshift mutations. A second process results in mutation is the induction of error-prone SOS repair when relatively high doses of mutagen are used.", "Image XResolution": "37", "Image YResolution": "37", "Image ResolutionUnit": "Pixels/Centimeter", "Image Software": "SlidePlayer", "Image YCbCrPositioning": "Centered"}