The differences in the median linking number of topoisomers isolated from S2 cells treated with RNAi to knockdown the indicated proteins were determined with respect to the linking number of plasmids isolated from cells in which MSL2, and therefore the MSL complex, was absent. (A) MOF knockdown, which leaves a complex of MSL1 + MSL2 + MLE, did not significantly change the topology compared with plasmid from compensated cells (GFP). MLE knockdown, which leaves a complex of MSL1 + MSL2, resulted in a partial or intermediate reduction in negative supercoiling of the plasmid. (B) ISWI knockdown produced more relaxed noncompensated and compensated topoisomers that migrated one linking number ahead of the similar plasmids from untreated cells. However, ADA3 and GCN5 knockdown in S2 cells did not significantly change the topology nor the migration of the isolated topoisomers.
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