ANORMAL KROMATİN PAKETLENMESİ IVF SONUÇLARINI ETKİLEMEKTEDİR Relationship between abnormal sperm chromatin packing and IVF results M.V. Filatov1,3, E.
{"Image DocumentName": "ANORMAL KROMAT\u0130N PAKETLENMES\u0130 IVF SONU\u00c7LARINI ETK\u0130LEMEKTED\u0130R", "Image ImageDescription": "Relationship between abnormal sperm chromatin packing and IVF results. M.V. Filatov1,3, E.V. Semenova1, O.A. Vorob eva2, O.A. Leont eva2 and E.A. Drobchenko Abstract. This study was initiated to determine the relationship between the fertilizing potential of spermatozoa and abnormalities in the compact packing of their chromatin which occurs in the final stage of male germ cell differentiation. Chromatin packing involves disulphide bridge covalent cross-linking. The degree of packing was determined from the accessibility of DNA to a fluorescent dye, ethidium bromide, following detergent treatment of the spermatozoa. The amount of dye bound was determined by flow cytometry in the presence or absence of heparin, a polyanion which removes only non-disulphide bridge-linked proteins. The results of a number of different sperm samples were compared with their results following in-vitro fertilization, and a relationship between disordered sperm chromatin packing and rates of embryo cleavage was observed. This study suggests that abnormal chromatin packing in spermatozoa may contribute to male fertility. Key words. chromatin packing ethidium bromide fertility disorder flow cytometry human spermatozoa.", "Image XResolution": "37", "Image YResolution": "37", "Image ResolutionUnit": "Pixels/Centimeter", "Image Software": "SlidePlayer", "Image YCbCrPositioning": "Centered"}