IV. results: step sizes of myosin V
{"Image DocumentName": "IV. results: step sizes of myosin V", "Image ImageDescription": "specific myosin V light chain domains targeted and labeled with single bifunctional rhodamine (BR) or monofunctional Cy3 (18.5 nm, 7 nm and 2.5 nm from midpoint of axis) labeled myosin V added to F-actin filaments immobilized on a coverslip and observation with TIRF. when no ATP present, no movement of dye spots observed; 300 nM of ATP allowed for visible step movement. 5,000 \u2013 10,000 photons per spot allow center location to 1.5 nm. 3 different myosin V step combinations observed: \uf0e0 74 nm steps (with in between 0 nm step) \uf0e0 alternating 52- & 23-nm steps. \uf0e0 alternating 42- & 33-nm steps.", "Image XResolution": "37", "Image YResolution": "37", "Image ResolutionUnit": "Pixels/Centimeter", "Image Software": "SlidePlayer", "Image YCbCrPositioning": "Centered"}