Micrographs of cultured OA chondrocytes labeled with iNOS antibody. (A) DW-DMEM, controls; (B) Incubation with IL-1β (5 ng/ml); (C) 25% or 50% VW-DMEM; (D) 25% or 50% VW-DMEM with IL-1β; (E) 100% VW-DMEM; (F) 100% VW-DMEM with IL-1β. The cells show a different immunolabelling pattern (arrows). Bar: 5 μm (from Fioravanti et al., 2013).
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