Flow Cytometric Evaluation of SVF
{"Image DocumentName": "Flow Cytometric Evaluation of SVF", "Image ImageDescription": "4 g adipose tissue digested in collagenase. Isolation of SVF via filtration and differential centrifugation. Accuri C6 flow cytometer and Beckman Coulter MoFlo XDP for FACS to identify and sort cell populations. PI stain to determine cell viability. Prepared cytospin preparation of the cells for cytologic identification. In order to preform flow cytometric analysis 4 grams of SQ adipose tissue was digested in a collagenase solution. The SVF was isolated using filtration and differential centrifugation. Isolated cells were then evaluated on the Beckman Coulter MoFlo flow cytometer for identification and sorting of cells. The isolated population of macrophages was stained with propidium idodide to determine cell viability and cytospin preparations of the cells were prepared to confirm cell identity.", "Image XResolution": "37", "Image YResolution": "37", "Image ResolutionUnit": "Pixels/Centimeter", "Image Software": "SlidePlayer", "Image YCbCrPositioning": "Centered"}