Thumbnail of West Nile virus (WNV) reverse transcription-polymerase chain reaction results from epizootic die-offs in farm-raised alligators. The expected amplicon is 248 bp. Lane 1, a 100-bp molecular weight ladder. Lane 2, the positive WNV control. Lane 3, fresh tissue samples from a juvenile alligator in the 2002 epizootic. Lane 4, virus isolation cell homogenate from a juvenile alligator in the 2002 epizootic. Lane 5, horsemeat that was being fed to alligators during the 2002 epizootic. Lane
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