NGP Antibody - Western Blot of rabbit anti-Ngp antibody. Lane 1: MW ladder (opal pre-stained). Lane 2: 32D lysate. Load: 10 µg per lane. Primary antibody: Ngp antibody at 1:1000 for overnight at 4°C. Secondary antibody: rabbit secondary HRP antibody at 1:70,000 for 45 min at RT. Block: BlockOut overnight at 4°C. Predicted/Observed size: 17, 20 kDa for Ngp. Higher banding due to glycosylation. ~125 kDa non-specific.
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