Figure 1. Deep imaging using simultaneous excitation at both 830 and 1100 nm with a Chameleon Discovery laser. The vessel wall of a mouse carotid artery showing en face and orthogonal views. The cyan structure shows collagen fibers (via second-harmonic generation [SHG] detection); the red are endothelial cell interfaces labeled with Alexa 647, and the green shows nuclei of the smooth muscle cells labeled with Cyto41. Courtesy of Dominic Depke, European Institute for Molecular Imaging (EIMI), Münster.
{}