Reversed Phase Chromatography
{"Image DocumentName": "Reversed Phase Chromatography", "Image ImageDescription": "Separation of ionic compounds - bases. pH increasing. pKa > pH. pKa \u2248 pH. pKa < pH. Highly polar (dissociated) analyte provides poor retention and peak shape. At pH similar to analyte pKa both, disociated and non-disociated forms are present, also ion interaction causes peak tailing. The peak is splitted and wide. WORST CASE! Non-disociated analyte provide better retention weak ion interaction still plays role (peak slightly tails). Sensitivity in ESI+ conditions (polarity in which most bases provide ions) could be lowered when high pH mobile phase is used!", "Image XResolution": "37", "Image YResolution": "37", "Image ResolutionUnit": "Pixels/Centimeter", "Image Software": "SlidePlayer", "Image YCbCrPositioning": "Centered"}