Flow cytometric analysis of U-2 OS cells, untreated (blue) or treated with DMOG (1 mM, 6 h; green), using HIF-1α (D1S7W) XP<sup>®</sup> Rabbit mAb (solid lines) or concentration-matched Rabbit (DA1E) mAb IgG XP<sup>®</sup> Isotype control #3900 (dashed lines). Anti-rabbit IgG (H+L), F(ab')<sub>2</sub> Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.
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