Méthodologie; Reconstitution et analyse de complexes H/ACA in vitro. Transcription/traduction in vitro ( 35 S-méthionine) NAF1 Dyskérine Fibrillarine
{"Image DocumentName": "\uf078 M\u00e9thodologie; Reconstitution et analyse de complexes H/ACA in vitro.", "Image ImageDescription": "Analyse surnageants. Gel d\u2019acrylamide ur\u00e9e. Gel d\u2019acrylamide. Bis-Tris 4-12% Transcription in vitro (32P-CTP) hTR204. hTR204 mut\u00e9s. U3 RNA CTRL- Purification sur gel. IP dysk\u00e9rine. ou NAF1. Transcription/traduction. in vitro (35S-m\u00e9thionine) NAF1. Dysk\u00e9rine. Fibrillarine CTRL- NHP2. NOP10. IP dysk\u00e9rine. ou NAF1. Gel d\u2019acrylamide. Bis-Tris 4-12% fixation, s\u00e9chage sur papier \u00e0 chromatographie et analyse sur un Molecular Imager FX. \uf078 Analyse des RNA sur Molecular Imager FX. 35S. transparent.", "Image XResolution": "37", "Image YResolution": "37", "Image ResolutionUnit": "Pixels/Centimeter", "Image Software": "SlidePlayer", "Image YCbCrPositioning": "Centered"}