(A) Microfluidic analyses of total RNA prepared from different life stages of Nylanderia pubens by the Trizol method. RNA standards and their corresponding sizes are provided in the left-most column. (B) PCR of a portion of the N. pubens ubiquin gene. DNase-treated RNA (from panel A) of larvae (lane 1 ) and adults (lane 2) was reverse transcribed and subsequently amplified by PCR. MW = molecular weight markers. Sizes shown on the left. (C) Results of spectrophotometric analysis of the RNA preparations from workers and larvae from the upper panel on an <t>ND-1000</t> spectrophotometer. Standard deviation values are indicated by error bars. The mean 260:280 nm ratio is provided for each life stage within the bar. High quality figures are available online.
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