ADENOSINE TRIPHOSPHATE (ATP) MEASUREMENT- FIREFLY METHODS ASTM D4012 Test Method for ATP Content of Microorganisms in Water ASTM D7463 Test method for Adenosine Triphospahate (ATP) content of Microorganisms in Fuel, Fuel/Water Mixtures and Fuel associated Water ASTM 7687-11 Standard Test Method for Measurement of Cellular Adenosine Thriphosphate in Fuel, Fuel/Water Mixtures, and Fuel Associated Water with Sample Concentration by Filtration The ATP firefly methods are rapid, sensitive determinations of viable microbial biomass.
{"Image DocumentName": "ADENOSINE TRIPHOSPHATE (ATP) MEASUREMENT- FIREFLY METHODS", "Image ImageDescription": "ASTM D4012 Test Method for ATP Content of Microorganisms in Water. ASTM D7463 Test method for Adenosine Triphospahate (ATP) content of Microorganisms in Fuel, Fuel/Water Mixtures and Fuel associated Water. ASTM Standard Test Method for Measurement of Cellular Adenosine Thriphosphate in Fuel, Fuel/Water Mixtures, and Fuel Associated Water with Sample Concentration by Filtration. The ATP firefly methods are rapid, sensitive determinations of viable microbial biomass. ATP is the primary energy donor for life processes, does not exist in association with nonliving material, and the amount of APT per unit of biomass (in weight) is relatively constant. The methods use the same scientific principle, but differ in several regards: D4012 and D7687 require filtration and reagent washes and extractions, by D7463 microbial ATP is captured, extracted in one step. D7687 differ from D4012 in that utilizes filtration and wash steps designed to eliminated interferences. D7687differ from D7463 in reporting : D7463 reports relative light units (RLU) consistent with method D4012, D7687 reports ATP concentration. D7687 can be used for detect cellular ATP in fuel and fuel stocks from which small quantities of water do not separate readily (ethanol blended gasoline containing >5%v/v), while D7463 can\u2019t be used for this type of conditions. D7687 measures cellular ATP in a single measurement, D7463 detects total ATP (as RLU) and extra cellular ATP (as RLU) using two separate analysis and calculates the cellular ATP (as RLU) as difference between the two. The APT Tests provide rapid test results that reflect the total bioburden in the sample. The results are available in matter of minutes, rather than 36 to 48h in the case of culture type testing.", "Image XResolution": "37", "Image YResolution": "37", "Image ResolutionUnit": "Pixels/Centimeter", "Image Software": "SlidePlayer", "Image YCbCrPositioning": "Centered"}