PCR Amplification of DNA Fragments Sticky-End PCR Method PCR 5'-CTAGC 3'-GATCG 5'-C 3'-G -3' -5' -3' -5' & Purify and mix 2 PCR products in an equimolar ratio; then de-nature and re-nature 5'-CTAGC -3' 3'-G -5' (Effective annealing PCR product for ligation) Primer #1 5'-CTAGC 5' # Primer #2 Primer #1 5'-CTAGC 5' # Primer #2 Separated PCR tube #1 Separated PCR tube #2 # Both reactions use the same reverse primer for PCR amplification.
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