SH induced G1/S cell cycle arrest in human breast cancer cells. ( a and b ) Cells were treated with SH for 48 h and subjected to DNA content analysis using a FACSCAN flow cytometer. ( c ) Proteins involved in G1/S transition were analyzed by western blotting. Cells were treated with SH (0, 0.25, 0.5 and 1.0 μ mol/ml) for 48 h, and total proteins were extracted. Equal protein loading was evaluated by β -actin. Data are represented as mean±S.D. of three independent experiments. * P
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