Induction of the qsd operon expression by 3-oxo-C 8 -HSL. qRT-PCR analysis of the qsdA , qsdC , and qsdR transcription was conducted in the R. erythropolis R138 wild-type strain grown in 7H9 medium in which 3-oxo-C 8 -HSL was added at mid-exponential phase. Expression levels of qsdA , qsdC , and qsdR are relative to those obtained in the same medium without the inducer. The dotted line indicates the same mRNA expression between the induced condition and without the inducers. Data shown are mean values obtained from three independent experiments. Statistical analysis was performed by the DataAssist TM software (v3.01) used for calculating relative quantitation of gene expression, based on the comparative C T (2 -ΔΔCT ) method. ∗ p -value
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