FIG. 5. RT-PCR detection of P2X7 mRNA in rat submandibular gland cells. The P2X7 transcript is expressed in the rat submandibular gland (SMG), and in both acinar (A) and ductal (D) cells purified from the same. RT-PCR detection of mRNA transcripts encoding this receptor is illustrated in an ethidium bromide-stained, 1.8% agarose gel of the corresponding PCR products. Rat whole brain (WB) RNA was used as a positive control. The ladder (f) is the fX174 HaeIII digest, with corresponding sizes in base pairs indicated to the left. The predicted size of the amplified product is 384 base pairs.
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