TcAUK1 localization in epimastigote forms. (A) Epimastigote forms were cell cycle synchronized with HU and the indicated cell cycle phases confirmed by flow cytometry. TcAUK1 (rabbit antiserum to TcAUK1) and nucleus/kinetoplast (DAPI). (B) TcAUK1 coding sequence was cloned into pTEXeGFP expression vector and epimastigotes were transfected. Shortly after transfection (24–48 hs), the localization of the fusion protein was evaluated by fluorescence microscopy.
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